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BIOINSECTICIDES AS FUTURE MAINSTREAM PEST CONTROL AGENTS: OPPORTUNITIES AND CHALLENGES

《农业科学与工程前沿(英文)》 2022年 第9卷 第1期   页码 82-97 doi: 10.15302/J-FASE-2021404

摘要:

Bioinsecticides are naturally-occurring substances from different sources that control insect pests. Ideal bioinsecticides should have low toxicity to non-target organisms. They should also be easily degraded in sewage treatment works and natural environments, highly effective in small quantities and affect target pests only. Public concerns about possible side-effects of synthetic pesticides have accelerated bioinsecticide research and development. However, to develop bioinsecticides into mainstream products, their high production costs, short shelf-life and often uncertain modes of action need to be considered. This review summarizes current progress on bioinsecticides which are categorized as biochemical insecticides and their derivatives, plant-incorporated protectants, and microbial bioinsecticides. The current constraints that prevent bioinsecticides from being widely used are discussed and future research directions are proposed.

 

关键词: biochemical insecticide / bioinsecticide / microbial bioinsecticides / plant-incorporated protectant / RNA insecticide    

ROUTE DEVELOPMENT, ANTIVIRAL STUDIES, FIELD EVALUATION AND TOXICITY OF AN ANTIVIRAL PLANT PROTECTANT

《农业科学与工程前沿(英文)》 2022年 第9卷 第1期 doi: 10.15302/J-FASE- 2021390

摘要:

It has previously been shown that tryptophan, the biosynthesis precursor of Peganum harmala alkaloids, and its derivatives have anti-TMV activity both in vitro and in vivo. Further exploration of this led to the identification of NK0238 as a highly effective agent for the prevention and control of diseases caused by plant viruses, but the existing routes are unsuitable for its large-scale synthesis. This study optimized a route for two-step synthesis of this virucide candidate via reaction of l-tryptophan with triphosgene to produce l-tryptophan-N-carboxylic anhydride, which then reacts with n-octylamine to give NK0238 at up to 94% yield and nearly 97% HPLC purity. In addition, the route was used for the preparation of NK0238 on a>40 g scale permitting further assessment of its antivirus activity in the greenhouse and field experiments, and toxicity tests. NK0238 exhibited useful antiviral activities against a variety of viruses both in greenhouse and field experiments. The toxicity tests showed that NK0238 was not acutely toxic to birds, fish, honey bees and silkworms. The optimized route provides a solid foundation for its large-scale synthesis and subsequent efficacy and toxicity studies, its excellent activity and safety make NK0238 a promising drug candidate for further development.

 

关键词: antiviral plant protectant / antiviral in the greenhouse / field evaluation / l-trp-NCA / synthesis optimization / toxicity tests    

ROUTE DEVELOPMENT, ANTIVIRAL STUDIES, FIELD EVALUATION AND TOXICITY OF AN ANTIVIRAL PLANT PROTECTANT

Wentao XU, Hao TIAN, Hongjian SONG, Yuxiu LIU, Yongqiang LI, Qingmin WANG

《农业科学与工程前沿(英文)》   页码 110-119 doi: 10.15302/J-FASE-2021390

摘要: It has previously been shown that tryptophan, the biosynthesis precursor of alkaloids, and its derivatives have anti-TMV activity both and . Further exploration of this led to the identification of NK0238 as a highly effective agent for the prevention and control of diseases caused by plant viruses, but the existing routes are unsuitable for its large-scale synthesis. This study optimized a route for two-step synthesis of this virucide candidate via reaction of L-tryptophan with triphosgene to produce L-tryptophan- -carboxylic anhydride, which then reacts with -octylamine to give NK0238 at up to 94% yield and nearly 97% HPLC purity. In addition, the route was used for the preparation of NK0238 on a > 40 g scale permitting further assessment of its antivirus activity in the greenhouse and field experiments, and toxicity tests. NK0238 exhibited useful antiviral activities against a variety of viruses both in greenhouse and field experiments. The toxicity tests showed that NK0238 was not acutely toxic to birds, fish, honey bees and silkworms. The optimized route provides a solid foundation for its large-scale synthesis and subsequent efficacy and toxicity studies, its excellent activity and safety make NK0238 a promising drug candidate for further development.

关键词: antiviral plant protectant     antiviral in the greenhouse     field evaluation     L-trp-NCA     synthesis optimization     toxicity tests    

Effect of wetland plant fermentation broth on nitrogen removal and bioenergy generation in constructedwetland-microbial fuel cells

《环境科学与工程前沿(英文)》 2022年 第16卷 第12期 doi: 10.1007/s11783-022-1592-x

摘要:

● Fermentation broth facilitates N removal and energy yields in tertiary CW-MFC.

关键词: Constructed wetland     Microbial fuel cell     Nitrogen removal     Bioenergy generation     Carbon source    

Plant diversity reduces the effect of multiple heavy metal pollution on soil enzyme activities and microbial

Yang GAO, Chiyuan MIAO, Jun XIA, Liang MAO, Yafeng WANG, Pei ZHOU

《环境科学与工程前沿(英文)》 2012年 第6卷 第2期   页码 213-223 doi: 10.1007/s11783-011-0345-z

摘要: It is unclear whether certain plant species and plant diversity could reduce the impacts of multiple heavy metal pollution on soil microbial structure and soil enzyme activities. Random amplified polymorphic DNA (RAPD) was used to analyze the genetic diversity and microbial similarity in planted and unplanted soil under combined cadmium (Cd) and lead (Pb) pollution. A metal hyperaccumulator, , and a common plant, , were used in this research. The results showed that microorganism quantity in planted soil significantly increased, compared with that in unplanted soil with Cd and Pb pollution. The order of microbial community sensitivity in response to Cd and Pb stress was as follows: actinomycetes>bacteria>fungi. Respiration, phosphatase, urease and dehydrogenase activity were significantly inhibited due to Cd and Pb stress. Compared with unplanted soil, planted soils have frequently been reported to have higher rates of microbial activity due to the presence of additional surfaces for microbial colonization and organic compounds released by the plant roots. Two coexisting plants could increase microbe population and the activity of phosphatases, dehydrogenases and, in particular, ureases. Soil enzyme activity was higher in phytoremediated soil than in planted soil in this study. Heavy metal pollution decreased the richness of the soil microbial community, but plant diversity increased DNA sequence diversity and maintained DNA sequence diversity at high levels. The genetic polymorphism under heavy metal stress was higher in phytoremediated soil than in planted soil.

关键词: enzyme activity     soil DNA     microbial population     plant diversity     heavy metal    

Rhizosphere immunity: targeting the underground for sustainable plant health management

Zhong WEI, Ville-Petri FRIMAN, Thomas POMMIER, Stefan GEISEN, Alexandre JOUSSET, Qirong SHEN

《农业科学与工程前沿(英文)》 2020年 第7卷 第3期   页码 317-328 doi: 10.15302/J-FASE-2020346

摘要:

Managing plant health is a great challenge for modern food production and is further complicated by the lack of common ground between the many disciplines involved in disease control. Here we present the concept of rhizosphere immunity, in which plant health is considered as an ecosystem level property emerging from networks of interactions between plants, microbiota and the surrounding soil matrix. These interactions can potentially extend the innate plant immune system to a point where the rhizosphere immunity can fulfil all four core functions of a full immune system: pathogen prevention, recognition, response and homeostasis. We suggest that considering plant health from a meta-organism perspective will help in developing multidisciplinary pathogen management strategies that focus on steering the whole plant-microbe-soil networks instead of individual components. This might be achieved by bringing together the latest discoveries in phytopathology, microbiome research, soil science and agronomy to pave the way toward more sustainable and productive agriculture.

关键词: rhizosphere     soil microbiome     plant immunity     microbial ecology     plant health     soilborne pathogens    

integrated-bioreactor with two zones treating odours from sludge thickening tank and dewatering house: performance and microbial

Jianwei Liu, Kaixiong Yang, Lin Li, Jingying Zhang

《环境科学与工程前沿(英文)》 2017年 第11卷 第4期 doi: 10.1007/s11783-017-0932-8

摘要: A full-scale integrated-bioreactor consisting of a suspended zone and an immobilized zone was employed to treat the ordours emitted from a wastewater treatment plant. The inlet concentrations of H S and NH were 1.6–38.6 mg·m and 0.1–6.7 mg·m , respectively, while the steady-state outlet concentrations were reduced to 0–2.8 mg·m for H S and 0–0.5 mg·m for NH . Both H S and NH were eliminated effectively by the integrated bioreactor. The removal efficiencies of H S and NH differed between the two zones. Four species of microorganisms related to the degradation of H S and NH were isolated. The characteristics and distributions of the microbes in the bioreactor depended on the inlet concentration of substrates and the micro-environmental conditions in the individual zones. Product analysis indicated that most of the H S was oxidized into sulfate in the immobilized zone but was dissolved into the liquid phase in the suspended zone. A large amount of NH was converted into nitrate and nitrite by nitration in the suspended zone, whereas only a small amount of NH was transferred to the aqueous phase mainly by absorption or chemical neutralization in the immobilized zone. Different microbial populations dominated the individual zones, and the major biodegradation products varied accordingly.

关键词: Biological deodorization     Microbial characteristics     Ammonia     Hydrogen sulfide     Wastewater treatment plant    

Ribozyme and the mechanisms that underlie RNA catalysis

Timothy J. Wilson,Yijin Liu,David M. J. Lilley

《化学科学与工程前沿(英文)》 2016年 第10卷 第2期   页码 178-185 doi: 10.1007/s11705-016-1558-2

摘要: Ribozymes are widespread, and catalyze some extremely important reactions in the cell. Mechanistically most fall into one of two classes, using either metal ions or general acid-base catalysis. The nucleolytic ribozymes fall into the latter class, mostly using nucleobases. A sub-set of these use a combination of guanine base plus adenine acid to catalyze the cleavage reaction. New ribozymes are still being discovered at regular intervals and we can speculate on the potential existence of ribozymes that catalyze chemistry beyond phosphoryl transfer reactions, perhaps using small-molecule coenzymes.

关键词: RNA catalysis     RNA structure     catalytic mechanism    

Taxonomic and functional variations in the microbial community during the upgrade process of a full-scalelandfill leachate treatment plant – from conventional to partial nitrification-denitrification

Binbin Sheng, Depeng Wang, Xianrong Liu, Guangxing Yang, Wu Zeng, Yiqing Yang, Fangang Meng

《环境科学与工程前沿(英文)》 2020年 第14卷 第6期 doi: 10.1007/s11783-020-1272-7

摘要: Abstract • Upgrade process was investigated in a full-scale landfill leachate treatment plant. • The optimization of DO can technically achieve the shift from CND to PND process. • Nitrosomonas was mainly responsible for ammonium oxidation in PND system. • An obviously enrichment of Thauera was found in the PND process. • Enhanced metabolic potentials on organics was found during the process update. Because of the low access to biodegradable organic substances used for denitrification, the partial nitrification-denitrification process has been considered as a low-cost, sustainable alternative for landfill leachate treatment. In this study, the process upgrade from conventional to partial nitrification-denitrification was comprehensively investigated in a full-scale landfill leachate treatment plant (LLTP). The partial nitrification-denitrification system was successfully achieved through the optimizing dissolved oxygen and the external carbon source, with effluent nitrogen concentrations lower than 150 mg/L. Moreover, the upgrading process facilitated the enrichment of Nitrosomonas (abundance increased from 0.4% to 3.3%), which was also evidenced by increased abundance of amoA/B/C genes carried by Nitrosomonas. Although Nitrospira (accounting for 0.1%–0.6%) was found to stably exist in the reactor tank, considerable nitrite accumulation occurred in the reactor (reaching 98.8 mg/L), indicating high-efficiency of the partial nitrification process. Moreover, the abundance of Thauera, the dominant denitrifying bacteria responsible for nitrite reduction, gradually increased from 0.60% to 5.52% during the upgrade process. This process caused great changes in the microbial community, inducing continuous succession of heterotrophic bacteria accompanied by enhanced metabolic potentials toward organic substances. The results obtained in this study advanced our understanding of the operation of a partial nitrification-denitrification system and provided a technical case for the upgrade of currently existing full-scale LLTPs.

关键词: Landfill leachate     Process upgrade     Partial nitrification-denitrification     Bacterial community     Metagenomics    

Digital microfluidics: A promising technique for biochemical applications

He WANG, Liguo CHEN, Lining SUN

《机械工程前沿(英文)》 2017年 第12卷 第4期   页码 510-525 doi: 10.1007/s11465-017-0460-z

摘要:

Digital microfluidics (DMF) is a versatile microfluidics technology that has significant application potential in the areas of automation and miniaturization. In DMF, discrete droplets containing samples and reagents are controlled to implement a series of operations via electrowetting-on-dielectric. This process works by applying electrical potentials to an array of electrodes coated with a hydrophobic dielectric layer. Unlike microchannels, DMF facilitates precise control over multiple reaction processes without using complex pump, microvalve, and tubing networks. DMF also presents other distinct features, such as portability, less sample consumption, shorter chemical reaction time, flexibility, and easier combination with other technology types. Due to its unique advantages, DMF has been applied to a broad range of fields (e.g., chemistry, biology, medicine, and environment). This study reviews the basic principles of droplet actuation, configuration design, and fabrication of the DMF device, as well as discusses the latest progress in DMF from the biochemistry perspective.

关键词: digital microfluidics     electrowetting on dielectric     discrete droplet     biochemistry    

Identifying human-induced influence on microbial community: A comparative study in the effluent-receiving

Yuhan Zheng, Zhiguo Su, Tianjiao Dai, Feifei Li, Bei Huang, Qinglin Mu, Chuanping Feng, Donghui Wen

《环境科学与工程前沿(英文)》 2019年 第13卷 第6期 doi: 10.1007/s11783-019-1174-8

摘要: Microbial compositions showed high differences in two study areas. COD was the key anthropogenic indicator in the coastal wastewater disposal area. Distinctive microbes capable of degrading toxic pollutants were screened. Microbial communities in effluent-receiving areas followed “niche theory”. Microbial community structure is affected by both natural processes and human activities. In coastal area, anthropegenetic activity can usually lead to the discharge of the effluent from wastewater treatment plant (WWTP) to sea, and thus the water quality chronically turns worse and marine ecosystem becomes unhealthy. Microorganisms play key roles in pollutants degradation and ecological restoration; however, there are few studies about how the WWTP effluent disposal influences coastal microbial communities. In this study, sediment samples were collected from two WWTP effluent-receiving areas (abbreviated as JX and SY) in Hangzhou Bay. First, based on the high-throughput sequencing of 16S rRNA gene, microbial community structure was analyzed. Secondly, several statistical analyses were conducted to reveal the microbial community characteristics in response to the effluent disposal. Using PCoA, the significant difference of in microbial community structure was determined between JX and SY; using RDA, water COD and temperature, and sediment available phosphate and ammonia nitrogen were identified as the key environmental factors for the community difference; using LDA effect size analysis, the most distinctive microbes were found and their correlations with environmental factors were investigated; and according to detrended beta-nearest-taxon-index, the sediment microbial communities were found to follow “niche theory”. An interesting and important finding was that in SY that received more and toxic COD, many distinctive microbes were related to the groups that were capable of degrading toxic organic pollutants. This study provides a clear illustration of eco-environmental deterioration under the long-term human pressure from the view of microbial ecology.

关键词: Microbial community structure     Effluent-receiving area     High-throughput sequencing     Costal sediments     Wastewater treatment plant (WWTP)    

Dissipation of polycyclic aromatic hydrocarbons and microbial activity in a field soil planted with perennial

Dengqiang FU, Ying TENG, Yuanyuan SHEN, Mingming SUN, Chen TU, Yongming LUO, Zhengao LI, Peter CHRISTIE

《环境科学与工程前沿(英文)》 2012年 第6卷 第3期   页码 330-335 doi: 10.1007/s11783-011-0366-7

摘要: Dissipation and plant uptake of polycyclic aromatic hydrocarbons (PAHs) in contaminated agricultural soil planted with perennial ryegrass were investigated in a field experiment. After two seasons of grass cultivation the mean concentration of 12 PAHs in soil decreased by 23.4% compared with the initial soil. The 3-, 4-, 5-, and 6-ring PAHs were dissipated by 30.9%, 25.5%, 21.2%, and 16.3% from the soil, respectively. Ryegrass shoots accumulated about 280 μg·kg , shoot dry matter biomass reached 2.48 × 10 kg·ha , and plant uptake accounted for about 0.99% of the decrease in PAHs in the soil. Significantly higher soil enzyme activities and microbial community functional diversity were observed in planted soil than that in the unplanted control. The results suggest that planting ryegrass may promote the dissipation of PAHs in long-term contaminated agricultural soil, and plant-promoted microbial degradation may be a main mechanism of phytoremediation.

关键词: perennial ryegrass     polycyclic aromatic hydrocarbon bioremediation     plant uptake     soil microbial activity    

Removal, distribution and plant uptake of perfluorooctane sulfonate (PFOS) in a simulated constructed

Weichuan Qiao, Rong Li, Tianhao Tang, Achuo Anitta Zuh

《环境科学与工程前沿(英文)》 2021年 第15卷 第2期 doi: 10.1007/s11783-020-1312-3

摘要: Abstract • PFOS was removed by soil adsorption and plant uptake in the VFCW. • Uptake of PFOS by E. crassipes was more than that of C. alternifolius. • PFOS in wastewater can inhibit the removal of nutrients. • Dosing with PFOS changed the soil microbial community in the VFCW. A vertical-flow constructed wetland (VFCW) was used to treat simulated domestic sewage containing perfluorooctane sulfonate (PFOS). The removal rate of PFOS in the domestic sewage was 93%–98%, through soil adsorption and plant uptake, suggesting that VFCWs can remove PFOS efficiently from wastewater. The removal of PFOS in the VFCW was dependent on soil adsorption and plant uptake; moreover, the percentage of soil adsorption was 61%–89%, and was higher than that of the plants uptake (5%–31%). The absorption capacity of Eichhornia crassipes (E. crassipes) (1186.71 mg/kg) was higher than that of Cyperus alternifolius (C. alternifolius) (162.77 mg/kg) under 10 mg/L PFOS, and the transfer factor of PFOS in E. crassipes and C. alternifolius was 0.04 and 0.58, respectively, indicating that PFOS is not easily translocated to leaves from roots of wetland plants; moreover, uptake of PFOS by E. crassipes was more than that of C. alternifolius because the biomass of E. crassipes was more than that of C. alternifolius and the roots of E. crassipes can take up PFOS directly from wastewater while C. alternifolius needs to do so via its roots in the soil. The concentration of 10 mg/L PFOS had an obvious inhibitory effect on the removal rate of total nitrogen, total phosphorus, chemical oxygen demand, and ammonia nitrogen in the VFCW, which decreased by 15%, 10%, 10% and 12%, respectively. Dosing with PFOS in the wastewater reduced the bacterial richness but increased the diversity in soil because PFOS stimulated the growth of PFOS-tolerant strains.

关键词: Vertical-flow constructed wetland     Perfluorooctane sulfonate     Wetland plants     Soil microbial community     Effect     Speciality: Wetlands     Transformation     Organic pollutants     Phytoremediation     Exposure assessment     Sewage    

Potential of electron transfer and its application in dictating routes of biochemical processes associated

《医学前沿(英文)》 2021年 第15卷 第5期   页码 679-692 doi: 10.1007/s11684-021-0866-1

摘要: Metabolic reprogramming, such as abnormal utilization of glucose, addiction to glutamine, and increased de-novo lipid synthesis, extensively occurs in proliferating cancer cells, but the underneath rationale has remained to be elucidated. Based on the concept of the degree of reduction of a compound, we have recently proposed a calculation termed as potential of electron transfer (PET), which is used to characterize the degree of electron redistribution coupled with metabolic transformations. When this calculation is combined with the assumed model of electron balance in a cellular context, the enforced selective reprogramming could be predicted by examining the net changes of the PET values associated with the biochemical pathways in anaerobic metabolism. Some interesting properties of PET in cancer cells were also discussed, and the model was extended to uncover the chemical nature underlying aerobic glycolysis that essentially results from energy requirement and electron balance. Enabling electron transfer could drive metabolic reprogramming in cancer metabolism. Therefore, the concept and model established on electron transfer could guide the treatment strategies of tumors and future studies on cellular metabolism.

关键词: metabolic reprogramming     potential of electron transfer     cell proliferation     aerobic glycolysis     cancer metabolism    

Proline-2′-deoxymugineic acid, a phytosiderophore analog, drives beneficial rhizobacterial community formation to promote peanut micronutrition

《农业科学与工程前沿(英文)》 doi: 10.15302/J-FASE-2023531

摘要:

● Proline-2′-deoxymugineic (PDMA) significantly altered the bacterial community in the peanut rhizosphere.

关键词: Beneficial rhizobacteria recruitment     peanut     plant-soil micronutrition enhancement     proline-2′-deoxymugineic acid     stable microbial network    

标题 作者 时间 类型 操作

BIOINSECTICIDES AS FUTURE MAINSTREAM PEST CONTROL AGENTS: OPPORTUNITIES AND CHALLENGES

期刊论文

ROUTE DEVELOPMENT, ANTIVIRAL STUDIES, FIELD EVALUATION AND TOXICITY OF AN ANTIVIRAL PLANT PROTECTANT

期刊论文

ROUTE DEVELOPMENT, ANTIVIRAL STUDIES, FIELD EVALUATION AND TOXICITY OF AN ANTIVIRAL PLANT PROTECTANT

Wentao XU, Hao TIAN, Hongjian SONG, Yuxiu LIU, Yongqiang LI, Qingmin WANG

期刊论文

Effect of wetland plant fermentation broth on nitrogen removal and bioenergy generation in constructedwetland-microbial fuel cells

期刊论文

Plant diversity reduces the effect of multiple heavy metal pollution on soil enzyme activities and microbial

Yang GAO, Chiyuan MIAO, Jun XIA, Liang MAO, Yafeng WANG, Pei ZHOU

期刊论文

Rhizosphere immunity: targeting the underground for sustainable plant health management

Zhong WEI, Ville-Petri FRIMAN, Thomas POMMIER, Stefan GEISEN, Alexandre JOUSSET, Qirong SHEN

期刊论文

integrated-bioreactor with two zones treating odours from sludge thickening tank and dewatering house: performance and microbial

Jianwei Liu, Kaixiong Yang, Lin Li, Jingying Zhang

期刊论文

Ribozyme and the mechanisms that underlie RNA catalysis

Timothy J. Wilson,Yijin Liu,David M. J. Lilley

期刊论文

Taxonomic and functional variations in the microbial community during the upgrade process of a full-scalelandfill leachate treatment plant – from conventional to partial nitrification-denitrification

Binbin Sheng, Depeng Wang, Xianrong Liu, Guangxing Yang, Wu Zeng, Yiqing Yang, Fangang Meng

期刊论文

Digital microfluidics: A promising technique for biochemical applications

He WANG, Liguo CHEN, Lining SUN

期刊论文

Identifying human-induced influence on microbial community: A comparative study in the effluent-receiving

Yuhan Zheng, Zhiguo Su, Tianjiao Dai, Feifei Li, Bei Huang, Qinglin Mu, Chuanping Feng, Donghui Wen

期刊论文

Dissipation of polycyclic aromatic hydrocarbons and microbial activity in a field soil planted with perennial

Dengqiang FU, Ying TENG, Yuanyuan SHEN, Mingming SUN, Chen TU, Yongming LUO, Zhengao LI, Peter CHRISTIE

期刊论文

Removal, distribution and plant uptake of perfluorooctane sulfonate (PFOS) in a simulated constructed

Weichuan Qiao, Rong Li, Tianhao Tang, Achuo Anitta Zuh

期刊论文

Potential of electron transfer and its application in dictating routes of biochemical processes associated

期刊论文

Proline-2′-deoxymugineic acid, a phytosiderophore analog, drives beneficial rhizobacterial community formation to promote peanut micronutrition

期刊论文